Download Chabot College Fall 2008 Replaced Fall 2011

Chabot College

Fall 2008 Replaced Fall 2011 Course Outline for Microbiology 1 MICROBIOLOGY

Catalog Description: 1 – Microbiology

5 units

Bacteria, fungi, protozoans, parasites, and viruses with an emphasis on their relationship to humans. Cultivation, control, metabolism, body's defense against disease, microbial genetics, laboratory tests, and contemporary diseases are discussed. Methods used in the laboratory include staining, investigation, cultivation, identification of unknowns, and sensitivity testing. Prerequisite: Biology 31, and Chemistry 30A or Chemistry 1A (both completed with a grade of "C" or higher). Strongly recommended: Anatomy 1, eligibility for English 1A . 3 hours lecture, 6 hours laboratory. Prerequisite Skills: Before entering the course, the student should be able to: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22, 23. 24.

describe and apply the scientific method and how it is used by scientists to further scientific knowledge; cite the characteristics and levels of organization exhibited by all living organisms; use the light microscope and dissecting scope; describe how cells/specialized cells are structured and function; describe basic cell metabolism; describe/contrast, mitosis and meiosis; describe structure, transmission and expression of genes; explain the Darwinian concept of evolution as modified by modern scientific knowledge; describe how the modern (binomial) system names and classifies organisms; make unit conversions in the metric system; describe the structure of the atom; perform calculations using the mole concept; identify properties of states of matter; write balanced equations for chemical reactions including those in aqueous solution and those involving elementary oxidation-reduction; describe properties of solutions; use Le Chatelier's principle to predict the qualitative effects of changes in concentration, temperature and pH on an equilibrium; perform laboratory experiments in an efficient, safe and purposeful manner; collect and analyze scientific data; perform titrations and volumetric analyses; write balanced chemical equations for oxidation-reduction reactions; calculate enthalpies of reaction using calorimetry, Hess's law, heats of formation and bond energies; describe the bonding in compounds and ions; explain chemical and physical changes in terms of thermodynamics; define all concentration units for solutions and solve solution stoichiometry problems.

1

Chabot College Course Outline for Microbiology 1, page 2 Fall 2008 Expected Outcome for Students: Upon completion of the course the student should be able to: 1. describe key aspects of the development of microbiology and its significance to modern microbiology; 2. handle microbial cultures in a safe manner; 3. demonstrate proficiency using the bright field microscope; 4. list the main characteristics and structural components of viruses, prokaryotic, and eukaryotic cells, and to discuss their functions; 5. purify and identify bacterial isolates based on metabolic profiles and microscopic characteristics; 6. explain the principles and interpretation of common bacteriological tests; 7. describe the role of fungi as pathogens and their importance in the environment; 8. investigate the antimicrobial susceptibility of bacterial isolates; 9. explain the use of disinfectants, antiseptics, sanitizers and the mode of action of selected examples; 10. explain fundamental bacterial genetic principles, and their relevance to disease development and biotechnology; 11. describe the main components and functions of the innate and acquire immunity; 12. describe the conditions that lead to infectious disease development in a population or an individual, and their control; 13. develop approaches to control the presentation of infectious diseases. Course Content (Lecture): 1.

2.

3.

Introduction to microbiology a. Areas of study in microbiology b. Taxonomy c. History Structure of bacteria a. Shapes and arrangements b. Flagella c. Fimbriae d. Capsules e. Cell wall f. Cell membrane g. Cytoplasmic inclusions h. Endospores Cultivation of bacteria a. Growth requirements b. Classification of media c. Obtaining and maintaining cultures

2

Chabot College Course Outline for Microbiology 1, page 3 Fall 2008 Course Content (Lecture) – continued: 4.

5.

6.

7.

8.

9.

10.

11.

Metabolism a. Enzyme structure and function b. Physical factors that influence enzyme activity c. Structure of carbohydrates, proteins, lipids, nucleic acids d. Metabolism of glucose, proteins, lipids e. Aerobic and anaerobic respiration f. ATP synthesis and function g. Structure of DNA and RNA h. Role of mRNA, tRNA, rRNA in protein synthesis i. Operon Model Genetics a. Mutations, i.e., chromosome and gene b. Genotype, phenotype c. Conjugation d. Transformation e. Transduction f. Genetic engineering Fungi a. Structure and reproduction of selected species b. Human diseases c. Growth requirements Viruses a. Composition b. Reproduction c. Animal viruses and diseases Rickettsiae a. Structure b. Vectors c. Human diseases d. Methods of diagnosis Control of Microorganisms a. Sterilization, disinfection, antimicrobial agents b. Physical factors c. Antibiotics and chemotherapeutic agents Immunology a. Nonspecific immunity, i.e., blood cells, lymphatic system, phagocytosis, tissue factors, complement b. Specific immunity, i.e., antigens, antibodies c. Clonal selection Theory d. T and B lymphocytes and their function Serological Diagnostic Methods a. Agglutination tests b. Precipitin tests c. Complement fixation tests d. Neutralization tests e. Radioimmunoassay testing f. Fluorescent antibody testing g. ELISA

3

Chabot College Course Outline for Microbiology 1, page 4 Fall 2008 Course Content (Lecture)-continued: 12.

Contemporary Diseases a. AIDS b. Infectious hepatitis c. Dental diseases

Course Content (Laboratory Activities): 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29.

Microscope Measuring cells Bacterial morphology and arrangements Aseptic and isolation techniques Staining procedures, i.e., simple, gram, acid-fast, spore, capsule Morphological unknown Anaerobic culture methods Carbohydrate fermentation Respiration reactions Protein utilization Differential media Enterotube II Bacterial unknown Serial dilutions and counting bacteria Tubidometry Effect of temperature Effect of U.V. Antibiotic testing-Kirby Bauer M.I.C. Testing Disinfectant testing Oral hygiene Evaluation of mouthwashes Throat culture Skin culture Protozoans Parasitic protozoans Parasitic flatworm Parasitic roundworms Arthropod vectors

Methods of Presentation: 1. 2. 3. 4. 5.

Lecture and discussion Observation, collection and analysis of data Laboratory studies Charts, models, slides, transparencies Films

4

Chabot College Course Outline for Microbiology 1, page 5 Fall 2008

Assignments and Methods of Evaluating Student Progress: 1.

Typical Assignments a. Lecture. Compare and contrast the cellular components of Gram positive and Gram negative bacteria. Discuss how these cells respond to antimicrobials in the context of their cellular structure b. Laboratory. Utilizing data gathered from the epidemiology exercise determine the rate of hand contact transmission of Serratia marcescens in a population. Plot the data in a chart and determine the prevalence of infection. Given an unknown culture determine by microscopy and biochemical tests the identity of the organisms. Proper use of aseptic technique and microscope use is assessed as well

2.

Methods of Evaluating Student Progress a. Examinations b. Quizzes c. Laboratory practical d. Bacterial unknowns e. Laboratory reports f. Final exam

Textbook(s) (Typical): Microbiology, 7th edition, Tortora, McMillian, 2007 Laboratory Experiments in Microbiology, Johnson, Pearson Benjamin Cummings, 2007 Special Student Materials: 1. 2. 3.

Lab coat or apron Marking pen Colored pencils

CE Revised 9/2007

5